The current research was conducted with the aim of analyzing the mediating role of organizational ambidexterity in the effect of corporate social responsibility and knowledge management infrastructure on sustainable performance. The research is based on the objective, applied, and in terms of method, survey. The statistical population was made up of all the managers and experts of the staff of Dr. Tamin Company, numbering 300 people. Based on the simple random sampling method and Cochran's formula, 168 people were selected as a sample. The data collection tool was a standard questionnaire whose validity was confirmed using the opinions of experts in the field of management and its reliability through Cronbach's alpha. Data were analyzed using structural equation model in SmartPLS3 software. The findings of the research showed that the value of the coefficient of the path of corporate social responsibility on organizational ambidexterity is equal to 0.417, with the T statistic (5.081); The value of the coefficient of the path of knowledge management infrastructure on organizational ambidexterity is equal to 0.379, the value of the coefficient of the path of social responsibility on sustainable performance is equal to 0.513, with the T statistic (11.980); The value of the path coefficient of knowledge management infrastructure on sustainable performance was equal to 0.439, the value of the path coefficient of organizational ambidexterity on sustainable performance was equal to 0.571, which were significant at the 95% confidence level. Also, the results showed that organizational ambidexterity can mediate the effect of social responsibility on sustainable performance to the extent of 0.751. In addition, organizational ambidexterity can mediate the effect of knowledge management infrastructure on sustainable performance to the extent of 0.655.